Is CD107a intracellular?

Is CD107a intracellular?

It is a widely expressed intracellular protein, located in the lysosomal/endosomal membrane. CD107a transiently located on the plasma membrane can be used as a marker for CD8+ T cell degranulation following stimulation.

What is CD107a?

Lysosomal-associated membrane protein 1 (LAMP-1) also known as lysosome-associated membrane glycoprotein 1 and CD107a (Cluster of Differentiation 107a), is a protein that in humans is encoded by the LAMP1 gene. The human LAMP1 gene is located on the long arm (q) of chromosome 13 at region 3, band 4 (13q34).

Is CD107a a surface marker?

In particular, CD107a/LAMP-1 has been widely used as a marker for degranulating cytotoxic lymphocytes, because surface expression is induced upon degranulation. CD107a is a highly glycosylated protein comprising a 40-kDa backbone with predicted 17 N-glycosylation and 9 O-glycosylation sites.

What is a degranulation assay?

A degranulation assay is based on flow cytometry, an thus this test can be combined with cellular phenotyping and analysis of effector molecule production (both cytokines and cytolytic components), for example, during characterization.

What is CD107a a marker for?

CD107a, also known as Lysosome associated membrane protein-1 (LAMP-1), is a marker for degranulation of NK cells and its expression correlates with NK cell-mediated lysis of target cells (Alter et al., 2004; Aktas et al., 2009).

What is degranulation of mast cells?

Degranulation is a cellular process that releases antimicrobial cytotoxic or other molecules from secretory vesicles called granules found inside some cells. It is used by several different cells involved in the immune system, including granulocytes (neutrophils, basophils, and eosinophils) and mast cells.

What is cd107 marker for?

CD107a as a functional marker for the identification of natural killer cell activity. J Immunol Methods.

What cell does perforin produce?

T lymphocytes
Perforin is a pore forming cytolytic protein found in the granules of cytotoxic T lymphocytes (CTLs) and natural killer cells (NK cells). Upon degranulation, perforin binds to the target cell’s plasma membrane, and oligomerises in a Ca2+ dependent manner to form pores on the target cell.

What is perforin quizlet?

Perforin. What is Perforin? A pore-forming molecule that allows granzymes to enter the target cell and trigger it into commiting suicide (apoptosis)

What happens in mast cell degranulation?

In the respiratory tract, mast cell degranulation increases vascular permeability and local edema, which can obstruct nasal airways and lead to congestion (9, 10). There is increased production of mucus and its accumulation can block off the sinuses and result in a bacterial infection.

What causes degranulation of mast cells?

The most significant means of causing such degranulation is via the interaction of allergen with mast cell-bound IgE molecules. A ‘sensitized’ mast cell is coated by allergen-specific IgE that affixes to the cell surface through interaction with the high-affinity Fce receptor (FceR type 1).

How is CD107a staining used in PBMCs?

CD107a staining can be performed simultaneously with intracellular cytokine staining by multi-parameter flow cytometry at an effector to target ratio of 10:1 To determine the optimal effector to target ratio for inducing maximal NK cell activation, PBMCs were stimulated with different concentrations of K562 target cells.

How are CD107a and B used to diagnose degranulation?

Thus, labeling responding cells with antibodies to CD107a and b and measuring their expression by flow cytometry can directly identify degranulating CD8+ T cells. The measurement of CD107 expression on the cell surface of activated T cells can be used with numerous other measures of T lymphocyte function.

Where is CD107a found on the cell surface?

CD107a is a type I, lysosomal membrane protein that is extensively glycosylated. It is expressed constitutively in the late endosomes-lysosomes in all cells. CD107a is also transiently expressed on the cell surface of degranulating cytolytic T cells.

How are CD107 and B expressed in CD8 + T cells?

CD107a and b are expressed on the cell surface of CD8+ T cells following activation with cognate peptide, concordant with production of intracellular IFNgamma. Finally, CD107-expressing CD8+ T cells are shown to mediate cytolytic activity in an antigen-specific manner.