What is the first strand cDNA synthesis kit?

What is the first strand cDNA synthesis kit?

The First Strand cDNA Synthesis Kit is used for the synthesis of the first strand cDNA as the starting reaction for two-step RT PCR.The kit includes Reverse Transcriptase AMV for first strand synthesis, two different primers, our PCR Nucleotide Mix, and Control Neo pa RNA.

Is there a cDNA synthesis kit for RT-PCR?

First Strand cDNA Synthesis Kit for RT-PCR (AMV). RT-qPCR, or quantitative reverse transcription PCR, combines the effects of reverse transcription and quantitative PCR or real-time PCR to amplify and detect specific targets.

How is a cDNA used as a template for a PCR?

cDNA Synthesis describes the generation of complementary DNA (cDNA) from an RNA template by reverse transcription. Reverse transcriptases (RTs) use an RNA template and a primer complementary to the RNA to direct the synthesis of the first strand cDNA, which can be used directly as a template for the Polymerase Chain Reaction (PCR).

What are the components of a qscript cDNA synthesis kit?

Both kits contain an RNase inhibitor protein. qScriptâ„¢ cDNA SuperMix is provided as a single tube reaction of 5X concentrated master mix. It provides all necessary components for first-strand synthesis including: buffer, dNTPs, MgCl2, primers, RNase inhibitor protein, qScript reverse transcriptase and stabilizers (except RNA template) .

https://www.youtube.com/watch?v=0_YmYSNPpfY

How big is a first strand cDNA reaction pack?

In addition, the 50-reaction pack size includes 10 control reactions. The Transcriptor First Strand cDNA Synthesis Kit provides all reagents required for first-strand cDNA synthesis reactions up to 14kb on all real-time instruments and conventional thermal cyclers. Three different priming methods can be used.

When to use reverse transcriptase in cDNA synthesis?

Due to its thermostability, Transcriptor Reverse Transcriptase is recommended for GC-rich templates with high secondary structure, without the need to include additives in the reaction. The kit provides all reagents required for first-strand cDNA synthesis reactions. For priming, three different primer systems can be used.