What is the purpose of using blood agar plates quizlet?
differential medium that distinguishes bacterial species by their ability to break down the red blood cells included in the media. Blood agar is often used to distinguish between the different species of pathogenic Streptococcus bacteria.
What is the principle of blood agar?
Principle of Blood Agar The blood added to the base provides more nutrition to the medium by providing additional growth factors required for these fastidious organisms. The blood also aids in visualizing hemolytic reactions of different bacteria.
What is the purpose of the agar?
Agar is commonly used in the laboratory to help feed and grow bacteria and other microorganisms. It acts as a culture that provides nutrients and a place for these items to grow, but since it is indigestible to the microorganisms, they cannot eat and destroy it.
What is purpose of blood in sheep blood agar plate quizlet?
Blood agar plate- tripticase soy agar plate containing 5% sheep red blood cells which supports the growth of most medically important bacteria and it is used to detect the hemolysis patterns of streptococci isolated from clinical specimens.
What is the purpose of using a blood agar plate?
Blood agar is a general purpose, enriched medium often used to grow fastidious organisms and to differentiate bacteria based on their hemolytic properties. In the U.S., blood agar is usually prepared from tryptic soy agar or Columbia agar base with 5% sheep blood.
Why do we use blood agar plates?
Blood Agar is used to grow a wide range of pathogens particularly those that are more difficult to grow such as Haemophilus influenzae, Streptococcus pneumoniae and Neisseria species. It is also required to detect and differentiate haemolytic bacteria, especially Streptococcus species.
Why is blood agar used for urine culture?
When a urine sample is submitted for culture, a calibrated loop is used to inoculate a blood agar plate, blood/MacConkey agar biplate (to help isolate gram negative bacteria) and CNA (Columbia Naladixic Acid) agar plate (to isolate gram positive bacteria). Cystocentesis samples will also be inoculated into thio broth.
Why sheep blood is used in blood agar?
Sheep blood has been preffered source in the Blood agar due to the fact that sheep RBCs are most sensitive to the the hemolytic toxins released by bacterial cells thus causing hemolytic zones around the colonies over the period of time.
What is the purpose of agar in a petri dish?
Agar plates are the standard solid support material for growing microorganisms. Microbial growth media contains nutrients and an energy source to fuel the microbes as they grow, and agar to keep the media in a semi-solid, gel-like state.
What is the purpose of the agar quizlet?
What is the purpose of Agar in the media? Help isolate organisms found in the minority; either enhance the growth of some organisms or inhibit the growth of other organisms. Contains chemicals that prevent the growth of unwanted bacteria without inhibiting the growth of the desired organism.
What are the nutrients in blood agar?
Although similar to the idea of nutrient agar media, Blood agar media contains five to ten percent sheep or rabbit blood. Blood agar composition consists of Beef extract (nitrogen source), Blood (nitrogen, amino acids, and carbon source), Sodium chloride (maintains osmotic balance), agar (solidifying agent).
Why is the streak plate preferred over spot inoculations for the blood agar plates?
why is streak plate preferred over spot inoculation in Blood Agar procedure? Quadrant streak is better for colony isolation as you start out with a much denser same and then isolate it down, making the test more reliable.
What is the purpose of the blood agar test?
Blood agar is used to support the growth of fastidious organisms and to determine the type of hemolysis (destruction of red blood cell walls) an organism produces.
What is the procedure for blood agar?
Procedure for the preparation of Blood Agar Prepare the blood agar base as instructed by the manufacturer. Sterilize by autoclaving at 121°C for 15 minutes. Transfer thus prepared blood agar base to a 50°C water bath. When the agar base is cooled to 50°C, add sterile blood aseptically and mix well gently. Avoid the formation of air bubbles.
What are the types of agar plates?
Types of agar plates Blood agar – contains blood cells from an animal (e.g. a sheep). Chocolate agar – this contains lysed blood cells, and is used for growing fastidious (fussy) respiratory bacteria. Neomycin agar – contains the antibiotic neomycin. Sabouraud agar – used for fungi.
Is blood agar is a selective or differential?
Blood Agar Is Not a Selective Medium. Blood agar is an enriched medium that provides an extra rich nutrient environment for microbes. Therefore, BAP is not a selective growth medium, since it supports the growth of a wide range of organisms.