Is Burkholderia cepacia Gram positive or negative?

Is Burkholderia cepacia Gram positive or negative?

Burkholderia cepacia is an aerobic gram-negative bacillus found in various aquatic environments. B cepacia is an organism of low virulence and is a frequent colonizer of fluids used in the hospital (eg, irrigation solutions, intravenous fluids). B cepacia rarely causes infection in healthy hosts.

Is Burkholderia Cenocepacia oxidase positive?

Out of 2079 cultured specimens, 150 isolates of non fermentative gram negative bacilli (NFGNB) were found to be oxidase positive. Only 91 of these oxidase positive isolates were able to grow on BCSA.

How do you identify Burkholderia cepacia?

For the laboratory diagnosis of B. cepacia, we recommend parallel cultures on blood agar medium and selective agar plates. Further identification of colonies with a Burkholderia phenotype should be performed with the API 20NE test.

Is Burkholderia cepacia catalase positive?

Burkholderia cepacia (B. cepacia), formerly known as Pseudomonas cepacia, is an aerobic, catalase-positive, gram-negative rod that was first isolated in 1950 as the responsible agricultural pest for onion skin rot.

What type of organism is Burkholderia?

Burkholderia cepacia [burk-hōld–er–ee-uh si–pay–shee–uh] (also called B. cepacia) is the name for a group or “complex” of bacteria that can be found in soil and water. B. cepacia bacteria are often resistant to common antibiotics.

What bacteria causes Burkholderia cepacia?

Burkholderia cepacia, a bacteria which used to be called Pseudomonas cepacia, is a rare but significant threat to people living with cystic fibrosis (CF). While the infection is most often symptom-free, it can turn serious, and even life-threatening.

Is Pseudomonas oxidase positive or negative?

Biochemical Test and Identification of Pseudomonas aeruginosa

Does Burkholderia cepacia ferment lactose?

Burkholderia cepacia complex (BCC), or simply Burkholderia cepacia, is a group of catalase-producing, lactose-nonfermenting, Gram-negative bacteria composed of at least 20 different species, including B.

Does Burkholderia cepacia grow on MacConkey Agar?

Burkholderia cepacia organisms grow well on standard solid media such as 5% sheep blood, chocolate, and MacConkey agars; in thioglycollate and brain-heart infusion broths; and in broth-based blood culture systems. On 5% sheep blood agar, B.

Where does Burkholderia cepacia come from?

cepacia outbreaks have been caused by contaminated medical products. This includes liquid docusate in 2016, contaminated mouthwash in 2005, and contaminated over-the-counter nasal spray in 2004. It can also be spread by person-to-person contact, contact with contaminated surfaces and exposure to B.

What is the structure of Burkholderia cepacia?

Burkholderia cepacia refers to a complex of nine closely related species listed above. They are rod-shaped, free-living, motile Gram- negative bacteria ranging from 1.6- 3.2 μm. They have been found to possess multitrichous polar flagella as well as pili used for attachment.

Is Burkholderia a pathogen?

Two of these species ( Burkholderia pseudomallei and Burkholderia mallei) are primary pathogens of animals and humans, two species ( Burkholderia caryophylli and Burkholderia gladioli) are known as plant pathogens, two species ( Burkholderia solanacearum [a plant pathogen] and Burkholderia pickettii [an opportunistic …

What are the biochemical characteristics of Burkholderia cepacia?

Some of the characteristics are as follows: Basic Characteristics Properties (Burkholderia cepacia) Bacteriocin Positive (+ve) Capsule Positive (+ve) Catalase Positive (+ve) Citrate Positive (+ve)

Which is the best selective media for b.cepacia?

B. cepacia complex grow well on standard laboratory media, and isolation from clinical specimens with mixed respiratory flora is enhanced by use of selective media, such as BCSA, OFPBL and PC agar ). Of these, BCSA agar is the most useful in supporting the growth of B. cepacia complex and suppressing the growth of other organisms.

What kind of disease does b.cepacia complex cause?

B. cepacia complex is an important cause of chronic respiratory infection among persons with cystic fibrosis ( 40) and of pneumonia, septicemia, and soft tissue abscesses among persons with chronic granulomatous disease ( 52 , 59 ).

How many genomovars are in the B cepacia complex?

Based on phenotypic and genotypic analyses, B cepacia is divided into 9 genomovars that constitute the B cepacia complex (BCC). B cepacia is almost always a colonizing organism rather than an infecting organism, but it may be important when isolated from body fluids that are ordinarily sterile.