How much should I load HyperLadder?

Vortex the tube and use 5 L per lane. There is no need to add loading buffer to the ladder. Use the 5x loading buffer for sample loading, under no circumstances should it be used for diluting the ladder. The blue dye in the loading buffer runs at approximately 4 bp on a 1% gel.

What is a HyperLadder?

Product Description. HyperLadder™ 1kb is our most popular molecular weight marker, composed of a restriction digest plus one or more PCR products, especially designed for easy size determination of linear double-stranded DNA fragments on 1% to 2% TAE or TBE agarose gels.

What is a HyperLadder gel electrophoresis?

HyperLadder™ 100bp is a molecular weight marker, composed of a restriction digest plus one or more PCR products, especially designed for easy size determination of linear double-stranded DNA fragments on 1% to 2% agarose gels.

Where on the gel will the largest DNA molecules be and why?

The largest fragments are near the top of the gel (negative electrode, where they began), and the smallest fragments are near the bottom (positive electrode).

How does SYBR Safe stain work?

SYBR Safe is a cyanine dye used as a nucleic acid stain in molecular biology. SYBR Safe binds to DNA. The resulting DNA-dye-complex absorbs blue light (λmax = 509 nm) and emits green light (λmax = 524 nm).

What is a DNA ladder used for?

A DNA ladder is a solution of DNA molecules of different lengths used in agarose or acrylamide gel electrophoresis. It is applied as a reference to estimate the size of unknown DNA molecules that were separated based on their mobility in an electrical field through the gel.

Where on the gel with the largest DNA molecules be and why quizlet?

Because each DNA molecule is negatively charged, it can be pulled through the gel by an electric field. Small DNA molecules move more quickly through the gel than larger DNA molecules. The bands closest to the start of the gel contain the largest DNA fragments.

Where will you find the largest DNA Where will you find the smallest DNA?

The bands furthest from the start of the gel contain the smallest fragments of DNA. The bands closest to the start of the gel contain the largest DNA fragments.

What is the function of SYBR Safe?

SYBR safe DNA gel stain is a highly sensitive stain for visualization of dna in agarose or acrylamide gels. SYBR safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide that can utilize either blue light or uv excitation.

How does SYBR Green II work?

SYBR Green II RNA gel stain is a sensitive nucleic acid gel stain that has bright fluorescence when bound to RNA and low background in gels, making it ideal for use with either formaldehyde/agarose or polyacrylamide gels using laser scanners or standard UV transilluminators.

What is a 1 kb DNA size standard ladder?

The 1 kb DNA ladder is a unique combination of a number of plasmids digested with restriction enzymes and PCR products to yield 13 DNA fragments that are suitable for use as a molecular weight standard for electrophoresis.

How to use hyperladder 1 KB molecular weight marker?

HyperLadder™ 1 kb is a popular, ready-to-use, molecular weight marker, specially engineered for easy size determination. The ready-to-use format reduces handling steps and saves time ; to use simply transfer HyperLadder™ 1 kb from the vial to the gel.

What can you do with a hyperladder 1kb?

With a wide size range, HyperLadder 1kb is perfect for size determination in techniques such as sequence analysis, confirmation of plasmid construction and PCR products as well as Southern blotting and other downstream techniques.

What is the mass of 1 kb DNA ladder?

1 kb DNA Ladder visualized by ethidium bromide staining on a 0.8% TAE agarose gel. Mass values are for 0.5 µg/gel lane. The following reagents are supplied with this product:

How long can you store 1 kb DNA ladder?

Use α- [ 32 P] dATP or α- [ 32 P] dTTP for the fill-in reaction. 1 kb DNA Ladder is stable for at least 3 months at 4°C. For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength.