What is a SphI?

Thermo Scientific PaeI (SphI) restriction enzyme recognizes GCATG^C sites and cuts best at 37°C in B buffer (Isoschizomers: BbuI, SphI). Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System.

What are the different types of restriction enzymes?

Today, scientists recognize three categories of restriction enzymes: type I, which recognize specific DNA sequences but make their cut at seemingly random sites that can be as far as 1,000 base pairs away from the recognition site; type II, which recognize and cut directly within the recognition site; and type III.

What are Neoschizomers and isoschizomers?

Isoschizomers are restriction enzymes that have the same recognition sequence and the same specificity. Neoschizomers recognize the same nucleotide sequence but cleave DNA at different positions.

What is an isoschizomers explain with example?

Isoschizomers are pairs of restriction enzymes specific to the same recognition sequence. For example, SphI (CGTAC/G) and BbuI (CGTAC/G) are isoschizomers of each other. For example, the restriction enzymes HpaII and MspI are isoschizomers, as they both recognize the sequence 5′-CCGG-3′ when it is unmethylated.

What is Type 3 restriction enzyme?

Type III restriction enzymes are a group of endonucleases that recognize a non-pallindromic sequence, comprising two inversely oriented sites.

What are isoschizomers and neoschizomers describe with relevant examples?

Isoschizomers are pairs of restriction enzymes specific to the same recognition sequence. For example, SphI (CGTAC/G) and BbuI (CGTAC/G) are isoschizomers of each other. Neoschizomers are a specific type (subset) of isoschizomer. For example, SmaI (CCC/GGG) and XmaI (C/CCGGG) are neoschizomers of each other.

What are neoschizomers examples?

Examples of neoschizomers are SmaI (5′-CCC↓GGG-3′) and XmaI (5′-C↓CCGGG-3′), which both recognize 5′-CCCGGG-3′ but cleave them differently and thus generate different types of ends (in this case, blunt ends for SmaI and 5′ protruding ends for XmaI).

What are isoschizomers used for?

Recognition site and cleavage specificity Isoschizomers are restriction enzymes that have the same recognition sequence and the same specificity. For instance, AgeI and BshT1 recognize and cleave 5′-A↓CCGGT-3′ in the same pattern.

What are Schizomers?

ISOSCHIZOMER • These are pairs of restriction enzymes specific to the same recognition recognition sequence. • For example, SphI (CGTAC/G) and BbuI (CGTAC/G) are isoschizomers of each other. • The first enzyme discovered which recognizes a given sequence is known as the prototype.

Which is E.coli strain carries the SPHI gene?

An E. coli strain that carries the SphI gene from Streptomyces phaeochromogenes (NRRL B-3559). One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Cleaves to leave a 3´ CATG extension which can be efficiently ligated to DNA fragments generated by NlaIII.

Which is the high fidelity version of SphI?

SphI has a High Fidelity version SphI-HF ® ( NEB #R3182 ). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.

How are proteins digested in the small intestine?

The two major pancreatic enzymes that digest proteins are chymotrypsin and trypsin. The cells that line the small intestine release additional enzymes that finally break apart the smaller protein fragments into the individual amino acids. The muscle contractions of the small intestine mix and propel the digested proteins to the absorption sites.

Which is a good source of protein for digestion?

Eggs are a good dietary source of protein and will be used as our example to describe the path of proteins in the processes of digestion and absorption. One egg, whether raw, hard-boiled, scrambled, or fried, supplies about six grams of protein. Figure 6.7 Digestion and Absorption of Protein