What is eFluor?

What is eFluor?

Invitrogen eBioscience eFluor organic dyes were developed specifically for labeling and detection of biomolecules. eFluor organic dyes are traditional fluorescent dyes for use in multiple applications, including multicolor flow cytometry and immunofluorescence microscopy.

How does Alexa Fluor work?

Alexa Fluor dyes can be conjugated directly to primary antibodies or to secondary antibodies to amplify signal and sensitivity or other biomolecules. The excitation and emission spectra of the Alexa Fluor series cover the visible spectrum and extend into the infrared.

What is PerCP in flow cytometry?

Peridinin Chlorophyll Protein Complex (PerCP)-conjugated antibodies are convenient tools for use in flow cytometry experiments. PerCP is a water soluble carotenoid pigment found in photosynthetic dinoflagellates.

What does Alexa Fluor bind to?

Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

Is PerCP a tandem dye?

These ready-made tandems of R-PE, PerCP and APC are optimized for brightness, spill-over and photostability.

Do antibodies bind to dead cells?

This is because dead cells have greater autofluorescence and increased non-specific antibody binding, which can lead to false positives and reduce the dynamic range.

Which dye is used for the staining dead cells?

2.6. Trypan blue is a stain used to quantify live cells by labeling dead cells exclusively. Because live cells have an intact cell membrane, trypan blue cannot penetrate the cell membrane of live cells and enter the cytoplasm. In a dead cell, trypan blue passes through the porous cell membrane and enters the cytoplasm.

What are the uses of eFluor organic dyes?

Invitrogen eBioscience eFluor organic dyes were developed specifically for labeling and detection of biomolecules. Each eFluor dye is engineered for superior optical performance in a variety of applications.

What kind of material is an EFluor Nanocrystal made of?

Unsourced material may be challenged and removed. eFluor nanocrystals are a class of fluorophores made of semiconductor quantum dots. The nanocrystals can be provided as either primary amine, carboxylate, or non-functional groups on the surface, allowing conjugation to biomolecules of a researcher’s choice.

Which is the brighter dye PerCP or eFluor?

PerCP-eFluor 710—a tandem dye excited with the blue (488 nm) laser; two to three-fold brighter than PerCP-Cy5.5. eFluor 660—an organic dye excited with the red (633 nm) laser; an alternative dye option to APC or Alexa Fluor®647. APC-eFluor 780—a tandem dye excited by the red (633 nm) laser; replaces APC-Alexa Fluor®750 in the eBioscience portfolio.

What is the stability of PerCP-eFluor 710 conjugates?

Stability testing of PerCP-eFluor 710 conjugates indicate stability as evidenced by minimal effects on compensation. PerCP-eFluor®710 is minimally affected by fixation or light exposure