What is m Enterococcus Agar?

Remel m-Enterococcus Agar is a solid medium recommended for selective isolation and enumeration of enterococci by direct plating or membrane filtration. SUMMARY AND EXPLANATION. The Enterococcus count is used as an indicator of sanitary quality in water and dairy products.

What is the differential ingredient in Slanetz and Bartley Agar?

Yeast extract is a source of vitamins, particularly of B-group. Glucose is the fermentable carbohydrate. Sodium phosphate acts as buffer. Sodium azide is the selective agent suppressing the growth of Gram-negative bacteria.

Is Enterococcus Agar selective or differential?

m-Enterococcus Agar is used for the selective isolation and enumeration of enterococci by membrane filtration in a laboratory setting. m-Enterococcus Agar is not intended for use in the diagnosis of disease or other conditions in humans.

What is Slanetz and Bartley medium?

Slanetz and Bartley Medium is used for the enumeration of enterococci from water samples as described in ISO 7899-2, and is not intended for use in the diagnosis of disease or other conditions in humans. This reaction is not exclusive to enterococci, and the count at this stage should be considered presumptive.

What test will differentiate an enterococcus from a streptococcus?

The bile-esculin test is widely used to differentiate enterococci and group D streptococci, which are bile tolerant and can hydrolyze esculin to esculetin, from non-group D viridans group streptococci, which grow poorly on bile.

What is the selective agent in bile esculin agar?

The first selective ingredient in this agar is bile, which inhibits the growth of Gram-positives other than enterococci and some streptococci species. The second selective ingredient is sodium azide. This chemical inhibits the growth of Gram-negatives. The differential ingredient is esculin.

How can you differentiate enterococci from Streptococcus pneumoniae?

Streptococci are gram-positive cocci arranged in pairs or chains. Streptococcus and Enterococcus species are catalase negative, which differentiates them from Staph- ylococcus, which is catalase positive. Streptococci have the typical gram-positive cell wall of peptidoglycan and teichoic acid and are nonmotile.

What test is used to differentiate Enterococcus faecalis from Streptococcus bovis?

bile-esculin test
The bile-esculin test is used to differentiate enterococci and group D streptococci from non-group D viridans group streptococci.

How does bile esculin test act as a selective and differential media?

Bile esculin agar is both selective and differential medium. The selective aspect of the bile esculin agar is sodium azide and oxagall which inhibit the growth of G- and most G+. The differential aspect of esculin agar is esculin which very few organisms can hydrolyze in the presence of bile.

What is the purpose of bile esculin agar?

Bile Esculin Agar is used primarily to differentiate Enterococcus from Streptococcus. Members of the genus Enterococcus are capable of growing in the presence of 40% bile (oxgall) and hydrolyzing esculin to glucose and esculetin. Esculetin combines with ferric ions to produce a black complex.

Which test would differentiate enterococci from Streptococcus?