Why do we use Sudan black stain?

Sudan black B was introduced as a specific fat stain for the detection of lipids in tissue sections by L. Saturated solutions of Sudan black B in 70% alcohol or in ethylene glycol stain the fat bodies of bacteria a deep blue-black color, and this dye is recommended as superior to the other Sudans.

How do you use Sudan black?

Staining Procedure:

Deparaffinize and hydrate to 70% alcohol.
Stand slides on end and allow to dry.
Place in Sudan black B solution for one to three hours.
Rinse thoroughly in two changes of 70% isopropyl alcohol.
Wash with six changes of distilled water.
Nuclear fast red solution for 3 minutes.

What does Sudan black bind to?

Sudan Black B (SBB) is a lipophilic dye that binds irreversibly to an undefined granule component in granulocytes, eosinophils and some monocytes. It cannot be extracted from the stained granules by organic dye solvents and gives comparable information to that of MPO staining.

What is the positive staining reaction of Sudan Black B?

Production of black and granular pigment indicates positive reaction. Lipids are present in azurophilic and secondary granules of myelocytic cells. Hence, these are SBB positive. The staining becomes more intense as cells mature from myeloblast to mature forms.

Why is aqueous mounting medium used in Sudan black?

Propylene glycol is the dye solvent so the dye solvent will not dissolve out any of the tissue lipid. Aqueous mounting media, such as glycerin jelly is used to mount the finished slide because the organic solvents present in synthetic resins would dissolve the dye-lipid complex.

What is the principle behind the Sudan test?

The Sudan IV test will test positive for lipids. The test procedure involves adding a few drops of Sudan IV to the test solution. Sudan IV is a dye that will stain lipids. If no lipids are present then the dye will sink to the bottom of the test tube.

How do you dissolve Sudan black?

Prepare a staining solution of 500 mg Sudan Black B in 20 ml of acetone. This is added to 15 ml of acetic acid, and then added to 85 ml of water. 2. Stir the mixture for 30 minutes and centrifuge to remove the precipitate.

Which fixative may be used for tissues to be stained with Sudan black?

10% formalin
An alternative stain to the Sudan Black B stain. CONTROL: Use a positive control of a fat smeared slide, and a negative control slide of a paraffin processed tissue, such as lung. FIXATIVE: 10% formalin. TECHNIQUE: Cut frozen tissue sections 10µ.

Will a Sudan black stain be positive or negative in all?

It showed common ALL antigen positive and were negative for myeloid antibodies. It is advisable to support the diagnosis of ALL in all cases with immunological markers.

What is the Colour of liquid droplets stain by Sudan Black B?

Saturated solutions of Sudan black B in 70% alcohol or in ethylene glycol stain the fat bodies of bacteria a deep blue-black color, and this dye is recommended as superior to the other Sudans.

Why is aqueous mounting medium used in this type of staining process?

Aqueous mounting medium are used for mounting sections from distilled water when the stains would be decolorized or removed by alcohol and xylene as would be the case with most of the fat stains (Sudan methods). Water (RI = 1.333).

What is aqueous mounting media?

The composition of the aqueous mounting media is based on distilled water. They are used to mount tissue sections directly after staining when the dye used is soluble in alcohol, xylene or other organic solvents such as most lipid dyes.

What is the principle of Sudan Black B stain?

Principle of Sudan Black B Stain As SBB is a fat soluble dye, it stains lipids such as sterols, neutral fats and phospholipids. These are present in azurophilic and secondary granules of myelocytic and lysosomal granules of monocytic cells. During staining, the dye leaves the solvent because of its high solubility in lipids than solvent.

What kind of dye is used for Sudan stain?

Sudan stain uses frozen tissue sections that are fixed using formalin solution or paraffinized sections. Sudan Black B dye is the most commonly used dye from the Sudan dye groups.

Which is better Sudan Black B or MPO?

Sudan Black B (SBB) is a fat soluble dye which has very high affinity for neutral fats and lipids. SBB staining is useful for for the differentiation of Acute myeloid leukemia (AML) from Acute lymphoid leukemia (ALL). It is similar to that of Myeloperoxidase (MPO) staining pattern of leukocytes and monocytes. It has few advantages over MPO:

How does Sudan Black B Treat autofluorescence?

Sudan Black B treatment reduces autofluorescence and improves resolution of in situhybridization specific fluorescent signals of brain sections Summary. Interference by autofluorescence is one of the major concerns of immunofluorescence analysis of in situ hybridization-based diagnostic assays.