Is DTT temperature sensitive?

Is DTT temperature sensitive?

DTT is hygroscopic and has sensitivity to heat. Incompatible with strong oxidizing agents, and decomposes at pH >7. 8. How long is it stable if shipped at room temperature?

Is DTT stable at?

In lyophilized form, the chemical is stable for 12 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles. Directions for Use: DTT is supplied as a lyophilized powder.

How long is DTT stable?

Storage and Stability A solution of DTT in Hepes buffer (pH 7.75) is stable for one week at +2 to +8°C if the container is tightly sealed and the solution is pro- tected from atmospheric oxygen by argon or nitrogen.

How long is DTT stable in solution at room temperature?

I found that, around this pH 8.5 in phosphate buffer stability of DTT is about 1.4 hours only. How much DNA template (genomic or plasmid DNA) is used for a general PCR?

Where do you store DTT?

Dissolve 1.5 g of DTT in 8 mL of H2O. Adjust the total volume to 10 mL, dispense into 1-mL aliquots, and store them in the dark (wrapped in aluminum foil) at -20°C (indefinitely). Do not autoclave DTT or solutions containing it.

Can DTT go bad?

If DTT is kept at room temperature it will degrade/oxidize. The main role of DTT is to keep proteins in a reduced state. If the DTT was left out for more than 72 hours it is definitely too late. Also it is better to store DTT at -20C for long term storage.

Can DTT be stored at room temp?

How do you store a DTT solution?

How do you store DTT?

Where do you store DTT powder?

Powder DTT should be stored at 4C however, 1M DTT solution should be stored at -20C.

What is the stability of dithiothreitol ( DTT )?

What is the stability of DTT? When refrigerated 2-8ºC, it is stable up to 3 years. DTT is hygroscopic and has sensitivity to heat. Incompatible with strong oxidizing agents, and decomposes at pH >7.

What are the side effects of exposure to dithiothreitol?

Can cause nausea, headache and vomiting. Exposure may result in blood in urine, difficulty breathing, irregular heartbeat, anemia, weakness, drunkenness, bluish skin color, lung congestion, kidney damage, paralysis, convulsions unconsciousness and coma.

Which is the oxidized form of dithiothreitol?

Dithiothreitol (DTT), also known as Cleland’s reagent, is a small-molecule redox reagent . Its oxidized form is a disulfide-bonded 6-membered ring. DTT has an epimeric (‘sister’) compound, dithioerythritol (DTE, #123378).

How long is DTT stable if shipped at room temperature?

When refrigerated 2-8ºC, it is stable up to 3 years. DTT is hygroscopic and has sensitivity to heat. Incompatible with strong oxidizing agents, and decomposes at pH >7. 8. How long is it stable if shipped at room temperature?