What is endA1?

What is endA1?

endA1: This strain lacks Endonuclease I (non-specific digestion) for cleaner preparations of DNA and better results in downstream applications. thi-1: This strain requires thiamine (thiamine auxotroph, cannot produce its own thiamine). recA1: RecA is a protein used by E. coli to repair and maintain DNA.

What is MG1655?

MG1655 is the strain chosen by the Blattner group for the first published sequence of a wild-type laboratory strain of E. coli K-12. Although MG1655 was chosen for having few genetic manipulations from the archetypal E. coli K-12 strain, most strains used in E.

What is the genotype of E. coli?

Genotypes of Invitrogen competent cells

What is the genotype of LE392?

Genotype: hsdR514(rk–, mk+), glnV(supE44), tryT (supF58), lacY1 or Δ(lacIZY)6, galK2, galT22, metB1, trpR55….What’s in the box?

What does endA1 mutation do?

The endA1 mutation inactivates an intracellular endonuclease to prevent it from degrading the inserted plasmid.

What is the purpose of subcloning?

Subcloning is a basic procedure in molecular biology for transfer of DNA inserts from one vector to another to gain functionality to study the sequence of interest.

What does K12 mean E coli?

E coli K12. E. coli K12, the prokaryotic wimp. Escherichia coli is a gram-negative bacterium that is a normal component of the human intestinal flora, and is ordinarily not a dangerous organism. The K12 and related strains of E.

What is the genome size of E coli mg1655?

4641652

How do you read e coli genotype?

Genes: In E. coli, the genotype only includes the genes that carry a loss of function mutation. The gene name is listed as three-letters in lower case and italics (DNA methylase is written as dam). Different genes affecting the same function/pathway are identified with different uppercase italic letters.

What is the role of CaCl2 in transformation?

The process of calcium chloride heat-shock transformation encourages bacterial cells to uptake DNA from the surrounding environment. The ice-cold CaCl2 solution facilitates binding of DNA to the surface of the cell, which then enters the cell after a short period of heat- shock (3).

What is subcloning vs molecular cloning?

The main difference between cloning and subcloning is that cloning is the production of clones of organisms or copies of cells or DNA fragments whereas subcloning is a technique used to move a particular DNA sequence from a parent vector to a destination vector.

What is the genotype of a person that has AB blood?

The blood type AB (also known as universal blood recipients and rarest blood type) has the genotype to be AB. The antigens are both A & B, and no antibodies in the blood plasma. The blood type O (also known as universal blood donors) has the genotype to be OO.

Why is AB negative the rarest blood type?

AB plasma is universal, and can be used for all patients regardless of their blood type. AB-negative is the rarest because it is only present in 1 out of 167 individuals. There are few combinations of alleles which can produce it, and the alleles are relatively rare.

What are facts about AB negative blood type?

A recipient with an AB negative blood type has both the antigens A and B on the blood but without the Rh antigen. Having the presence of A and B antigens and the absence of the Rh antigen, AB negative blood type can only receive blood types from AB-, B-, A- and O-.

What are the characteristics of blood type AB?

The blood type AB, meaning it has an antigen A and B but does not have antibodies A and B. People with AB Blood type are also called to have a strong and the patient feeling of empathy even slightly closed. In addition, they are also often referred to as humanists because they use more logic than feelings.