What is Fura Red?
Fura Red is a visible light-excitable fura-2 analog that offers unique possibilities for ratiometric measurement of calcium ion in single cells by microphotometry, imaging or flow cytometry when used with single excitation, green-fluorescent calcium indicators.
How does Fura-2 work?
Fura-2, an aminopolycarboxylic acid, is a ratiometric fluorescent dye which binds to free intracellular calcium. The use of the ratio automatically cancels out confounding variables, such as variable dye concentration and cell thickness, making Fura-2 one of the most appreciated tools to quantify calcium levels.
How do you dissolve fluo 4 am?
Dissolve Fluo-4 AM in 25 µl of DMSO. After adding DMSO, vortex tube briefly to dissolve the indicator dye, then centrifuge briefly to collect all contents at the tube bottom. Add entire contents of indicator dye tube to assay buffer solution to make a dye loading solution.
What is the meaning of Fura?
Fura means millet balls, which means that the millet is grounded into a powdered form, rolled and molded into balls to make the Fura, so also the Fura is mashed and added to Nono which is made from fermented milk.
What is a ratiometric dye?
Ratiometric, or dual-wavelength, ion indicators are a subcategory of fluorescent dyes utilized for their ability to quantitatively measure intracellular ion concentrations.
Why is Fura-2 unable to cross cell membranes?
Fluorescent Dyes – Loading Calcium indicators are unable to cross lipid membranes due to their nature, making necessary the use of physical or chemical methods to load them inside the cell. This is the case of Indo-1 AM and Fura-2 AM, which are fluorescent and calcium insensitive.
What does GCaMP stand for?
genetically encoded calcium indicator
GCaMP is a genetically encoded calcium indicator (GECI) initially developed in 2001 by Junichi Nakai. It is a synthetic fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin light-chain kinase.
What is Fura called in English?
The meaning of Fura de nunu in English is simply milk and millet gruel. Fura da Nono as the Hausas call it or Fura de nunu as the majority of Nigerians choose to refer to it, is a very healthy and satisfying food. Nono or Nunu is a local yogurt with thick lumps produced from the fermentation of cow milk.
What is Fura made from?
The fura balls are made from millet flour which has been spiced with ginger, cloves and sometimes, pepper. It is a very healthy and satisfying dish.
What does ratiometric mean?
In electronic or electromechanical systems, it refers to the output voltage as a ratio of the supply voltage. For example, if the input voltage is doubled, the output voltage is doubled.